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KMID : 0374019850080020099
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Ewha Medical Journal
1985 Volume.8 No. 2 p.99 ~ p.109
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Effect of Vitamin A and E on the Interaction Between 2-Acetylaminofluorene and Rat Liver DNA in Vivo
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Hong Young-Sook
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Abstract
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Binding of the hepatocarcinogen 2 acetylaminofluorene (AAF) to the DNA of rat liver homogenates was examined in vivo. Animals fed 1)w and high concentrations vitamin A and E for 28 days and DNA isolated 2 hr following a single
i.p. injection of 2 [9-14C~ acetylaminofluorene. The precipitated DNA was dissol- ´. ved in MUP, blended and passed through an HAP column. UVabsorbing material eluted with 0.48M Nap. Following dialysis, the fraction was enzymatically hydrolyzed purified by Sephadex LH-20 chromatography and the adducts were separated by HPLC. The ability of vitamin A and E on the binding of AAF to liver DNA in vivo was markedly inhibited the formation of the proximate carcinogen, N (deoxyguanosine- NZ - yl) AAF (C-- 8 adduct). Vitamin A at higher concentration(2,000LU.) exerted a stronger inhibitory effect on the formation C8 adducts. A similar inhibition pattern was observed in higher concentration vita-min ´E administered rats.
The data presented here demonstrated marked inhibits in the pattern of C8 deoxyguanosine adduct formation during the premaligment phase of 2AAF carcinogenes is.
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